Phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone.
Identifieur interne : 002D45 ( Main/Exploration ); précédent : 002D44; suivant : 002D46Phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone.
Auteurs : R. Welle [Allemagne] ; H. GrisebachSource :
- Archives of biochemistry and biophysics [ 0003-9861 ] ; 1989.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Acyltransferases (métabolisme), Cellules cultivées (MeSH), Chalcone (analogues et dérivés), Chalcone (biosynthèse), Chalcones (MeSH), Dosage immunologique (MeSH), Focalisation isoélectrique (MeSH), Immunodiffusion (MeSH), Induction enzymatique (effets des médicaments et des substances chimiques), NADP (pharmacologie), Oxidoreductases (analyse), Oxidoreductases (biosynthèse), Oxidoreductases (métabolisme), Plantes (enzymologie), Propiophénones (biosynthèse), Soja (MeSH), Sérums immuns (MeSH), Technique de Western (MeSH).
- MESH :
- analogues et dérivés : Chalcone.
- analyse : Oxidoreductases.
- biosynthèse : Chalcone, Oxidoreductases, Propiophénones.
- effets des médicaments et des substances chimiques : Induction enzymatique.
- enzymologie : Plantes.
- métabolisme : ARN messager, Acyltransferases, Oxidoreductases.
- pharmacologie : NADP.
- Cellules cultivées, Chalcones, Dosage immunologique, Focalisation isoélectrique, Immunodiffusion, Soja, Sérums immuns, Technique de Western.
English descriptors
- KwdEn :
- Acyltransferases (metabolism), Blotting, Western (MeSH), Cells, Cultured (MeSH), Chalcone (analogs & derivatives), Chalcone (biosynthesis), Chalcones (MeSH), Enzyme Induction (drug effects), Immune Sera (MeSH), Immunoassay (MeSH), Immunodiffusion (MeSH), Isoelectric Focusing (MeSH), NADP (pharmacology), Oxidoreductases (analysis), Oxidoreductases (biosynthesis), Oxidoreductases (metabolism), Plants (enzymology), Propiophenones (biosynthesis), RNA, Messenger (metabolism), Soybeans (MeSH).
- MESH :
- chemical , analogs & derivatives : Chalcone.
- chemical , analysis : Oxidoreductases.
- chemical , biosynthesis : Chalcone, Oxidoreductases, Propiophenones.
- chemical , metabolism : Acyltransferases, Oxidoreductases, RNA, Messenger.
- drug effects : Enzyme Induction.
- enzymology : Plants.
- chemical , pharmacology : NADP.
- Blotting, Western, Cells, Cultured, Chalcones, Immune Sera, Immunoassay, Immunodiffusion, Isoelectric Focusing, Soybeans.
Abstract
Chromatofocusing on Mono P proved to be an efficient purification procedure for the NADPH-dependent reductase from soybean (Glycine max L.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). By isoelectric focusing the pI of reductase was determined to be 6.3. Addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (Phaseolus vulgaris) and from young flowers of Dahlia variabilis caused in each case synthesis of 6'-deoxychalcone. When 4-coumaroyl-CoA was replaced by caffeoyl-CoA in the reductase assay, formation of 2',4',3,4-tetrahydrochalcone (butein) was observed. A polyclonal antireductase antiserum was raised in rabbits and proved to be specific in Ouchterlony diffusion experiments, Western blots and immunotitration. The reductase antiserum showed no cross-reactivity with soybean chalcone synthase (CHS). A biotin/[125I]streptavidin system provided a quantitative Western blot for the reductase. Changes in the activities, amounts of protein, and mRNA activities of reductase and CHS were determined after challenge of soybean cell cultures by elicitor (from Phytophthora megasperma f.sp. glycinea or yeast). For both enzymes a pronounced and parallel increase in activity and amounts of protein was observed after elicitor addition with a maximum at about 16 h after challenge. Parallel increases in mRNA activities occurred earlier. The results indicate a parallel induction of de novo synthesis of reductase and CHS which coact in synthesis of 6'-deoxychalcone.
DOI: 10.1016/0003-9861(89)90199-9
PubMed: 2500065
Affiliations:
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Grisebach</name></author></analytic><series><title level="j">Archives of biochemistry and biophysics</title><idno type="ISSN">0003-9861</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyltransferases (metabolism)</term><term>Blotting, Western (MeSH)</term><term>Cells, Cultured (MeSH)</term><term>Chalcone (analogs & derivatives)</term><term>Chalcone (biosynthesis)</term><term>Chalcones (MeSH)</term><term>Enzyme Induction (drug effects)</term><term>Immune Sera (MeSH)</term><term>Immunoassay (MeSH)</term><term>Immunodiffusion (MeSH)</term><term>Isoelectric Focusing (MeSH)</term><term>NADP (pharmacology)</term><term>Oxidoreductases (analysis)</term><term>Oxidoreductases (biosynthesis)</term><term>Oxidoreductases (metabolism)</term><term>Plants (enzymology)</term><term>Propiophenones (biosynthesis)</term><term>RNA, Messenger (metabolism)</term><term>Soybeans (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Acyltransferases (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Chalcone (analogues et dérivés)</term><term>Chalcone (biosynthèse)</term><term>Chalcones (MeSH)</term><term>Dosage immunologique (MeSH)</term><term>Focalisation isoélectrique (MeSH)</term><term>Immunodiffusion (MeSH)</term><term>Induction enzymatique (effets des médicaments et des substances chimiques)</term><term>NADP (pharmacologie)</term><term>Oxidoreductases (analyse)</term><term>Oxidoreductases (biosynthèse)</term><term>Oxidoreductases (métabolisme)</term><term>Plantes (enzymologie)</term><term>Propiophénones (biosynthèse)</term><term>Soja (MeSH)</term><term>Sérums immuns (MeSH)</term><term>Technique de Western (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Chalcone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oxidoreductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Chalcone</term><term>Oxidoreductases</term><term>Propiophenones</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyltransferases</term><term>Oxidoreductases</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Chalcone</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Chalcone</term><term>Oxidoreductases</term><term>Propiophénones</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Enzyme Induction</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Induction enzymatique</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Acyltransferases</term><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>NADP</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>NADP</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Cells, Cultured</term><term>Chalcones</term><term>Immune Sera</term><term>Immunoassay</term><term>Immunodiffusion</term><term>Isoelectric Focusing</term><term>Soybeans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Chalcones</term><term>Dosage immunologique</term><term>Focalisation isoélectrique</term><term>Immunodiffusion</term><term>Soja</term><term>Sérums immuns</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chromatofocusing on Mono P proved to be an efficient purification procedure for the NADPH-dependent reductase from soybean (Glycine max L.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). By isoelectric focusing the pI of reductase was determined to be 6.3. Addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (Phaseolus vulgaris) and from young flowers of Dahlia variabilis caused in each case synthesis of 6'-deoxychalcone. When 4-coumaroyl-CoA was replaced by caffeoyl-CoA in the reductase assay, formation of 2',4',3,4-tetrahydrochalcone (butein) was observed. A polyclonal antireductase antiserum was raised in rabbits and proved to be specific in Ouchterlony diffusion experiments, Western blots and immunotitration. The reductase antiserum showed no cross-reactivity with soybean chalcone synthase (CHS). A biotin/[125I]streptavidin system provided a quantitative Western blot for the reductase. Changes in the activities, amounts of protein, and mRNA activities of reductase and CHS were determined after challenge of soybean cell cultures by elicitor (from Phytophthora megasperma f.sp. glycinea or yeast). For both enzymes a pronounced and parallel increase in activity and amounts of protein was observed after elicitor addition with a maximum at about 16 h after challenge. Parallel increases in mRNA activities occurred earlier. The results indicate a parallel induction of de novo synthesis of reductase and CHS which coact in synthesis of 6'-deoxychalcone.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2500065</PMID><DateCompleted><Year>1989</Year><Month>07</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-9861</ISSN><JournalIssue CitedMedium="Print"><Volume>272</Volume><Issue>1</Issue><PubDate><Year>1989</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Archives of biochemistry and biophysics</Title><ISOAbbreviation>Arch Biochem Biophys</ISOAbbreviation></Journal><ArticleTitle>Phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone.</ArticleTitle><Pagination><MedlinePgn>97-102</MedlinePgn></Pagination><Abstract><AbstractText>Chromatofocusing on Mono P proved to be an efficient purification procedure for the NADPH-dependent reductase from soybean (Glycine max L.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). By isoelectric focusing the pI of reductase was determined to be 6.3. Addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (Phaseolus vulgaris) and from young flowers of Dahlia variabilis caused in each case synthesis of 6'-deoxychalcone. When 4-coumaroyl-CoA was replaced by caffeoyl-CoA in the reductase assay, formation of 2',4',3,4-tetrahydrochalcone (butein) was observed. A polyclonal antireductase antiserum was raised in rabbits and proved to be specific in Ouchterlony diffusion experiments, Western blots and immunotitration. The reductase antiserum showed no cross-reactivity with soybean chalcone synthase (CHS). A biotin/[125I]streptavidin system provided a quantitative Western blot for the reductase. Changes in the activities, amounts of protein, and mRNA activities of reductase and CHS were determined after challenge of soybean cell cultures by elicitor (from Phytophthora megasperma f.sp. glycinea or yeast). For both enzymes a pronounced and parallel increase in activity and amounts of protein was observed after elicitor addition with a maximum at about 16 h after challenge. Parallel increases in mRNA activities occurred earlier. The results indicate a parallel induction of de novo synthesis of reductase and CHS which coact in synthesis of 6'-deoxychalcone.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Welle</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II der Universität, Freiburg, Federal Republic of Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grisebach</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Arch Biochem Biophys</MedlineTA><NlmUniqueID>0372430</NlmUniqueID><ISSNLinking>0003-9861</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D047188">Chalcones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007106">Immune Sera</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011427">Propiophenones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>53-59-8</RegistryNumber><NameOfSubstance UI="D009249">NADP</NameOfSubstance></Chemical><Chemical><RegistryNumber>5S5A2Q39HX</RegistryNumber><NameOfSubstance UI="D002599">Chalcone</NameOfSubstance></Chemical><Chemical><RegistryNumber>B9CTI9GB8F</RegistryNumber><NameOfSubstance UI="C040920">isoliquiritigenin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.-</RegistryNumber><NameOfSubstance UI="D000217">Acyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.3.1.74</RegistryNumber><NameOfSubstance UI="C020141">flavanone synthetase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000217" MajorTopicYN="N">Acyltransferases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002599" MajorTopicYN="N">Chalcone</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D047188" MajorTopicYN="N">Chalcones</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004790" MajorTopicYN="N">Enzyme Induction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007106" MajorTopicYN="N">Immune Sera</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007118" MajorTopicYN="N">Immunoassay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005779" MajorTopicYN="N">Immunodiffusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007525" MajorTopicYN="N">Isoelectric Focusing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010944" MajorTopicYN="N">Plants</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011427" MajorTopicYN="N">Propiophenones</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1989</Year><Month>7</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1989</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1989</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2500065</ArticleId><ArticleId IdType="pii">0003-9861(89)90199-9</ArticleId><ArticleId IdType="doi">10.1016/0003-9861(89)90199-9</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><noCountry><name sortKey="Grisebach, H" sort="Grisebach, H" uniqKey="Grisebach H" first="H" last="Grisebach">H. Grisebach</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Welle, R" sort="Welle, R" uniqKey="Welle R" first="R" last="Welle">R. Welle</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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